Fusarium head blight(FHB) of wheat is mainly caused by Fusarium graminearum. It results in yield reduction and deteriorates seed quality. As a new monocotyledonous model plant, Brachypodium distachyon(Bd21) is able to interact with F. graminearum but often shows relatively resistant phenotype at normal growth condition. In this paper, we studied the progress of F. graminearum against B. distachyon 21 spikes at different conditions and confirmed the optimal condition for type Ⅱ resistance evaluation at 28℃, ...