【摘要】 利用硅胶柱分离瑞香狼毒乙酸乙酯萃取物得到Ⅰ、Ⅱ、Ⅲ段粗分物,并测定对东亚飞蝗毒杀活性最高的I段粗分物对东亚飞蝗的致死性及对其体内Na⁺-K⁺-ATPase、Ca²⁺/Mg²⁺-ATPase、乙酰胆碱酯酶的抑制活性.研究表明:瑞香狼毒I段粗分物对东亚飞蝗具有较强的毒杀作用,在药物浓度为10mg/mL、72h对东亚飞蝗的校正死亡率为92.50%;同时,毒杀作用具有浓度和时间依赖性;而且瑞香狼毒乙酸乙酯萃取物I段粗分物对上述3种酶均有一定的抑制作用,且对Na⁺-K⁺-ATPase的抑制作用最强,在药物浓度为5mg/mL、96h对Na⁺-K⁺-ATPase的抑制率为40.38%;分析发现瑞香狼毒活性成分对东亚飞蝗的毒杀作用与对Na⁺-K⁺-ATPase的抑制作用之间具有显著相关性,据此推测,Na⁺-K⁺-ATPase可能是瑞香狼毒活性成分的主要作用靶标之一.更多还原

【Abstract】 Using silica gel column to separate the ethyl acetate extract of Stellera chamaejasme L.we can getⅠ,Ⅱ,Ⅲsections.The activities of Na⁺-K⁺-ATPase、Ca²⁺/Mg²⁺-ATPase and acetylcholinesterase of insects were determined in vivo.The results showed that I section fromS.chamaejasme L.had the strongest poison effect on Locusta migratoria manilensis（Meyen）.Under the concentration of 10 mg/mL,the corrected mortality was 92.50%in 72 hour.And the poison effect there was persistence.The I section fromS.chamaejasme L.also had certain inhibition on activity of Na⁺-K⁺-ATPase、CCa²⁺/Mg²⁺-ATPase、acetylcholinesterase.The inhibiton rate of Na⁺-K⁺-ATPase was the highest among the three kinds of enzymes.There was a well correlation between the toxic action of the active ingredients fromS.chamaejasme L.to L.migratoria manilensis（Meyen）and the inhibition on activity of Na⁺-K⁺-ATPase in L.migratoria manilensis（Meyen）.Na⁺-K⁺-ATPase is likely the target of the active ingredients from S.chamaejasme L.against L.migratoria manilensis（Meyen）.更多还原