【摘要】 以唐古特大黄为材料,采用匀浆涂布法进行内生放线菌的分离,并运用平板对峙法和生长速率法筛选内生放线菌的抑菌活性;皿内抑菌结果表明,菌株4-21对稻瘟病菌抑制率高达84.12%.通过形态学观察、生理生化特性检测和16SrDNA序列分析将菌株4-21鉴定为Streptomyces albidoflavus.通过盆栽试验证明,菌株4-21对水稻稻瘟病具有良好的防治效果,其相对防治效果为52.73%.结果表明,内生放线菌4-21在水稻稻瘟病的生物防治中具有潜在的应用价值.更多还原

【Abstract】 Endophytic actinomycetes were isolated fromRheum tanguticum by spread plate method.Inhibitory activities of endophytic actinomycetes were determined by dual-culture inhibition on agar plate and growth rate method.As a result,Strain 4-21 with high inhibitory effect on Magnaporthe grisea was found.The results of the inhibiting effects experiment indicated that the strain 4-21 had the strong antagonistic effects,with the inhibition rates of Magnaporthe grisea was 84.12%.The strain 4-21 was indentified as Streptomyces albidoflavus according to the morphology biochemical characterization and 16 SrDNA gene sequence analysis.The results of potted control effect test showed that the biocontrol efficacy of strain 4-21 to Magnaporthe grisea was 52.73%.Strain 4-21 deserved to be developed a kind of biocontrol agent.更多还原