【摘要】 建立了电阻抗法快速测定巴氏杀菌牛乳中菌落总数的方法。得到的阻抗检测时间与菌落总数的标准曲线方程为y=-0.5923t+9.4172,相关系数r=-0.9907,可信度高;其可信检测限为10~107m L-1,完全满足食品安全国家标准的要求;设立的样品评价标准可以快速、直观的对巴氏牛乳样品进行评价,在7.46 h内即可快速的判定样品是否合格,大大缩短了检测周期。结果表明,与国标法相比,电阻抗法简便快速、灵敏度高,又准确可靠,且适用于不同时期、来源及酸度的产品,具有广泛的适用性和实用性。更多还原

【Abstract】 A method for rapid detection of aerobic plate count in pasteurized cow’s milk by impedance method was established. The following relationship between the germ count and the impedance detection time(t) was determined for the calibration equation: y=-0.5923 t+9.4172, r=-0.9907,with high reliability, and the authentic detection threshold was 10~107m L-1, fully meet the requirements of the national food safety standard. The establishment of the sample evaluation criteria could be fast, intuitive evaluation of the milk samples, the fastest 7.46 h could determine whether the sample is qualified, greatly shorten the detection time. Compared with the national standard method, the method was simple, rapid, sensitive, accurate and reliable, and could be used in different periods, sources and acidity of the product, with a wide range of applicability and practicality.更多还原