【摘要】 【目的】观察β-细辛醚对A549、PC3、PC9-R 3种肿瘤细胞增殖活力、周期、凋亡及迁移的作用,为β-细辛醚应用于肿瘤治疗提供实验依据。【方法】将不同浓度的β-细辛醚分别作用于A549、PC3、PC9-R肿瘤细胞24 h、48 h及72 h后,采用CCK-8法检测各细胞光密度(D)值,计算细胞增殖活力,采用流式细胞术测定各细胞DNA周期及细胞凋亡率,采用Transwell小室检测细胞迁移。【结果】不同浓度β-细辛醚作用A549、PC3、PC9-R肿瘤细胞24 h、48 h及72 h后,与正常对照组比较,3种细胞生长均有下降趋势,呈浓度依赖性和时间依赖性,细胞周期均表现为G0/G1期细胞比例显著上升,S期细胞比例和增殖指数显著降低,细胞凋亡率显著增高,细胞迁移显著减少(均P<0.05或P<0.01)。【结论】β-细辛醚可抑制A549、PC3、PC9-R肿瘤细胞的生长,阻滞细胞从G0/G1期进入S期,抑制DNA的合成,促进细胞凋亡,抑制细胞迁移。更多还原

【Abstract】 Objective To observe the effect of β-asarone on the proliferation,cycle,apoptosis and migration of tumor cells A549,PC3,and PC9-R,thus to provide experimental basis for the application of β-asarone to the t reatment of cancer. Methods After A549, PC3, and PC9-R were cultured with different concentrations ofβ-asarone for 24 h,48 h,and 72 h respectively,CCK-8 was used to detect the optical density(D) of cell proliferation,and then the cell proliferation rate was calculated. The flow cytometry was used to measure the cell DNA cycle and cell apoptosis, and Transwell Chambers were used to detect the cell migration. Results After treatment with different concentrations of β-asarone for 24 h, 48 h, and 72 h respectively, the growth of A549,PC3,and PC9-R showed declining trend in concentration-and time-dependent manner. The proportion of A549,PC3,and PC9-R at G0/G1 phase was increased,the proportion of the three kinds of cells at S phase and the proliferation indexes were declined,the apoptotic rate of A549, PC3,and PC9-R was increased,and the migration of A549,PC3,and PC9-R was reduced(P < 0.05 or P < 0.01 compared with those of the normal control group). Conclusion β-asarone has certain effects on suppressing proliferation, blocking G0/G1 phase developing into S phrase, inhibiting DNA synthesis, promoting the apoptosis, and inhibiting the migration of A549,PC3 and PC9-R.更多还原