【摘要】 目的探讨纳米儿茶素-Fe²⁺对人心血管平滑肌细胞的基因转染效率及细胞毒性,为后续对其转染机制及转染条件优化的研究提供依据。方法制备纳米儿茶素-Fe²⁺的基因导入材料,将质量比为50∶1的儿茶素-Fe²⁺/pEGFP-C1复合物转染人心血管平滑肌细胞,并设Lipo2000为对照,在荧光显微镜下观察细胞转染情况,采用MTT法检测儿茶素-Fe²⁺/pEGFP-C1复合物转染对人心血管平滑肌细胞生长的影响,评价细胞毒性。结果纳米儿茶素-Fe²⁺与pEGFP-C1最佳结合效率为50∶1;纳米儿茶素-Fe²⁺组与Lipo2000组转染率差异无统计学意义（P>0.05）;儿茶素-Fe²⁺纳米粒子对转染细胞无明显细胞毒性;儿茶素-Fe²⁺/pEGFP-C1纳米粒子转染后细胞荧光及EGFP荧光强度与Lipo2000组比较,转染效率相当（P>0.05）。结论儿茶素-Fe²⁺是一种低细胞毒性,高转染率的纳米材料,具备良好的应用前景。更多还原

【Abstract】 Objective To explore the novel catechin-Fe²⁺nanoparticle system,and evaluate its toxicity and transfectionefficiency on vascular smooth muscle cells. Methods The novel catechin-Fe²⁺nanoparticle system was constructed. TheDNA-binding efficiency of catechin-Fe²⁺nanoparticle,catechin-Fe²⁺and pEGFP-C1 plasmid DNA were determined by use ofgel retardation assay. Based on the results of gel retardation assay,the ratio of 50∶1 was considered as the optimal massratio. Catechin-Fe²⁺/pEGFP-C1 nanoparticles complex were transfected into vascular smooth muscle cells and cells transfectedwith pEGFP-C1 using lipofectamine2000 was served as a control. Cell transfection was observed under fluorescentmicroscope,and influence of lentivirus transfection on proliferation of vascular smooth muscle cells was detected by MTT.Results The optimal mass ratio for atechin-Fe²⁺and atechin-Fe²⁺is 50∶1. There was no statistical significant differencebetween the catechin-Fe²⁺transfected and lipofectamine 2000 transfected cells（P>0.05）. Atechin-Fe²⁺nanoparticles is nosignificant cell toxicity to transfected cells. Moreover, the cell fluorescence and EGFP fluorescent intensity detected byflow cytometry in the catechin-Fe²⁺/pEGFP-C1 nanoparticles transfected cells was as high as lipofectamine 2000 transfectedcells（P>0.05）. Conclusion Catechin-Fe²⁺nanoparticles is a promising system with high transfection efficiency and littletoxicity.更多还原