【摘要】 目的比较不同分期及分化程度的乙肝相关肝细胞癌中成纤维细胞激活蛋白(FAP)的表达,探讨成纤维细胞激活蛋白在肝癌中的表达差异及其意义。方法采用酶联免疫吸附方法检测成纤维细胞激活蛋白在不同分期及分化程度的乙肝相关肝细胞癌中的表达水平;用免疫组化方法对成纤维细胞激活蛋白的组织分布进行定位检查。结果成纤维细胞激活蛋白在不同分期的乙肝相关肝细胞癌组织1μg总蛋白中平均含量分别为Ⅰ期(1 039.82±248.89)pg/mL,Ⅱ期(1 173.57±184.60)pg/mL,Ⅲ期及以上(1 344.69±225.47)pg/mL,对照组为(742.15±443.16)pg/mL,差异有统计学意义(F=13.818,P=0.000);在不同分化程度的肝细胞癌组织中分别为高分化(1 008.18±148.20)pg/mL,中分化(1 126.52±218.38)pg/mL,低分化(1 414.96±311.94)pg/mL,差异有统计学意义(F=13.368,P=0.000)。免疫组化结果证实肝癌细胞中有成纤维细胞激活蛋白的表达,且其含量与肝癌细胞的病理分化程度具有明显相关性,随着肝癌细胞由高分化向低分化发展,成纤维细胞激活蛋白的表达也逐渐增加。结论成纤维细胞激活蛋白在不同分期及分化程度的乙肝相关肝细胞癌中表达存在差异,分期越晚,分化程度越低,成纤维细胞激活蛋白表达越多。更多还原

【Abstract】 ObjectiveTo analyze the expression of fibroblast activation protein(FAP)in different stages and different differentiation of hepatitis B-related hepatocellular carcinoma and discuss its clinical significance.Methods 47 hepatitis B-related hepatocellular carcinoma patients who underwent surgical resection were included.Enzyme-linked immunosorbent assay and immunohistochemical staining were used to measure fibroblast activation proteinconcentrations and to locate its expression.17 normal liver tissues were also included for comparison.Results In different stages of hepatitis B-related hepatocellular carcinoma,the values of fibroblast activation protein in 1μg total protein content for stageⅠ,ⅡandⅢwere(1 039.82±248.89),(1 173.57±184.60)and(1 344.69±225.47)pg/mL,respectively,while the normal control group was(742.15±443.16)pg/mL.The differences among the four groups were statistically significant(F=13.818,P=0.000).In different differentiation of hepatitis B-related hepatocellular carcinoma,the values of Fibroblast activation protein for well-differentiated,differentiated and poorly differentiated were(1 008.18±148.20),(126.52±218.38)and(1 414.96±311.94)pg/mL,respectively;the differences were also statistically significant(F=13.368,P=0.000).Immunohistochemical staining confirmed that fibroblast activation protein expressed in hepatic cell carcinoma;the intensity was significantly correlated with the degree of cancer cells′differentiation.Conclusion There were differences in the expression of fibroblast activation protein in different stages and differentiation of hepatitis B-related hepatocellular carcinoma;as the disease progresses,the expression of fibroblast activation protein was progressive.更多还原