【摘要】 目的探讨花色苷对CCl₄导致肝纤维化模型小鼠的改善作用,并初步探讨其抗纤维化的作用机制。方法将30只Balb/c小鼠随机分为空白对照组、CCl₄组和CCl₄+花色苷组。空白对照组给予生理盐水灌胃和玉米油腹腔注射,CCl₄组给予生理盐水灌胃和溶于玉米油的10%CCl₄腹腔注射,花色苷组给予10μg/g的花色苷灌胃和溶于玉米油的10%CCl₄腹腔注射。结合常规HE、苦味酸-天狼猩红染色评价肝组织炎症及纤维化程度,Western blot和q-PCR检测肝脏α-平滑肌肌动蛋白（α-SMA）、Ⅰ型胶原蛋白（COL1A1）以及转化生长因子-β1（TGF-β1）的表达水平,并通过激光共聚焦检测肝星状细胞自噬水平。结果花色苷干预组与CCl₄组相比纤维化指标检测均有降低且差异具有统计学意义（P<0.05）,肝星状细胞的活化与自噬水平受到抑制。结论花色苷能够改善CCl₄所致肝纤维化水平,可能与降低肝星状细胞的自噬水平有关。更多还原

【Abstract】 Objective To observe the intervention effect and mechanism of anthocyanin on the CCl₄ induced hepatic fibrosisin rat models. Methods 30 mice were randomly divided into normal control group（group A）,CCl₄ group（group B）,CCl₄ +anthocyanin group（group C）,and there were 10 mice in each group. These groups were separately established byintraperitoneal injection of normal saline,carbon tetrachloride（CCl₄ ） and CCl₄ ,respectively. Group A and B wereintragastric administrated with normal saline for group A and B,and group C with anthocyanin. The effect on the fibrosisdegrees of hepatic tissues was evaluated by routine pathological HE and Sirius Red Staining. The expression levels of α-SMA,COL1A1 and TGF-β1 were detected using western blot and q-PCR,and the autophagy level of hepatic stellate cell wasevaluated through the laser confocal microscope. Results Compared with CCl₄ group,the fibrosis and autophagy indexes ofgroup C were both significantly lower（P<0.05）. Conclusion Anthocyanin could reduce the liver fibrosis level induced byCCl₄ ,which might due to the suppression of the activation of hepatic stellate cell and the formation of autophagosome.更多还原