【摘要】 目的研制一种纳米级的超声造影剂,并评价其体内外超声显像的效果。方法利用薄膜-超声法制备纳米级超声造影剂,通过正交试验设计筛选出最佳处方。制作质量分数1%琼脂糖凝胶孔洞模型,以磷酸盐缓冲液为阴性对照,评价自制超声造影剂的显像效果。选择4T1荷瘤小鼠为研究对象,尾静脉注射造影剂24 h后,对其肿瘤部位进行超声成像分析。结果通过正交试验筛选出的最佳处方为以蛋黄卵磷脂和大豆卵磷脂质量比1∶1作为膜材料,全氟己烷的体积分数为3%,制备得到的造影剂粒径为(304.0±19.2)nm,多分散指数(PDI)为0.171±0.032,且在10 d内较稳定;体外显像实验发现造影剂可以显著增强回声信号;体内超声显像实验发现造影剂可以大量累积到肿瘤内部,同时增强肿瘤内部的回声信号。结论自制的超声造影剂性质稳定,超声显像效果好,为肿瘤的超声诊断与治疗相结合奠定基础。更多还原

【Abstract】 Objective To develop a nanometer ultrasound contrast agent, and evaluate its enhanced effect of ultrasound image in vitro and in vivo. Methods The nanometer ultrasound contrast agent was prepared by the thin film ultrasonic method, and the optimum formulation was optimized by orthogonal design. The 1% agarose gel pore model was used to evaluate the enhanced effect of ultrasound image of the nanometer ultrasound contrast agent with phosphate buffer used as a negative control. 4 T1 tumor bearing mice were selected for in vivo ultrasound imaging. After 24 h of intravenous injection, the ultrasound image of the tumor site was obtain. Results The optimum formulation was that the weight fraction of egg yolk lecithin and soybean lecithin was 1∶1 and the concentration of perfluorohexane was 3%. The particle size of prepared nanometer ultrasound contrast agent was(304.0±19.2) nm with the polydispersity index(PDI) of(0.171±0.032), which was relatively stable after the storage for ten days at 4 °C. The in vitro ultrasound imaging showed that the contrast agent can significantly enhance echo signal. In vivo ultrasound imaging showed that the contrast agent could accumulate in the tumor sites and enhance the echo signal. Conclusion The self-made nanometer ultrasound contrast agent is stable, having good performance in ultrasound imaging, which lays the foundation for the ultrasonic diagnosis and therapy of tumor.更多还原