Piled alkaline keratinase unhairing method is used on rabbit skin in this article. The dosage of keratinase,dosage of lime and temperature are optimized. The best processing parameters for rabbit skin unhairing are obtained. At the same time, the rabbit hair treated with lime and enzymes are analyzed with SEM and DSC, in order to determine the effect of enzyme unhairing on hair. The results show that, the hair of rabbit can be effectively removed in 4 h, when the dosage of enzyme is 0.05%-0.09%, temperature...