Three typical neutral protease preparations were purified through gel filtration chromatographywith the Superdex 75 prep grade column, and the proteins with different molecular weight were separated. The molecular weight values of the obtained active enzyme proteins were determined by SDS-PAGE electrophoresis, and their activities toward casein, collagen and elastin were also evaluated. Finally, the effects of the three enzyme activity components on hair-removal and grain-damage were investigated.The result...