水稻(Oryza sativa)起源于热带,随着人们对粮食需求的增长其种植区不断向北扩展,这就要求选择耐冷能力强的水稻品种。但是目前,对水稻耐冷性的分化及其机制的研究尚少见报道。本研究以辽宁、江苏和广东3个不同气候地区水稻为实验材料,通过冷害症状及叶绿素荧光指数比较水稻耐冷性差异,采用DNA甲基化亚硫酸盐测序法(bisulfite sequencing PCR,BSP),研究CBF(C-repeat binding factor)冷响应转录通路的ICE1(inducer of CBF expression 1)基因区域与启动子区域甲基化水平影响不同品种水稻冷害差异的分子机制。结果表明ICE1基因区域甲基化水平无差异,但是启动子区域甲基化有显著差异(P<0.05)。辽宁丹粳17(LN004)的ICE1基因启动子区域2个胞嘧啶被甲基化,耐冷指数最高;江苏的南粳5055(JS013)的ICE1基因启动子区域7个胞嘧啶被甲基化,耐冷指数居中;广东的粤新占2号(GD008)的ICE1基因启动子区域11个胞嘧啶被甲基化,耐冷指数为最低。定量PCR检测结果显示,ICE1和其下游调控的CBF1(C-repeat DRE bi...
【英文摘要】
Rice(Oryza sativa) is originated from the tropics, but human is continuously exploring its suitable planting areas northward, thus, the selection of cold-tolerant rice cultivars is required. However, the studies on the mechanism of differentiation on cold tolerance among rice cultivars has not been documented. In the present study, three materials of rice germ from different climate regions in Liaoning, Jiangsu and Guangdong provinces in China were selected. Their cold-tolerance was comparatively determined...
植物的耐冷性是限制植物生长发育和地理分布的重要决定因子,低温会造成植物不同程度的冷害,甚至死亡(段俊枝等,2014)。低温冷害对水稻(Oryza sativa)生产的影响是一个全球性的问题,比较严重的地区主要集中在南亚、东南亚的高海拔或高纬度地区(Baruah et al.,2009;黄力士等,2008