目的观察水蛭素对喉癌荷瘤小鼠的抑瘤作用。方法将40只小鼠随机分为4组,每组10只,小鼠左前腋窝下皮下种瘤建立实体瘤模型,阳性对照组以氟尿嘧啶20mg/kg腹腔注射;水蛭素高剂量组以2.5g/(kg·d)灌胃;阴性对照组以生理盐水0.1m L/(10g·d)灌胃;水蛭素低剂量组以1.2g/(kg·d)灌胃。连续用药14 d后处死小鼠取荷瘤鼠肿块称重,并将瘤组织包埋、固定、切片、染色等,计算抑瘤率,利用Image-Pro Plus 6.0图像分析软件检测瘤组织中C-met的表达。结果水蛭素高、低剂量组对小鼠喉癌细胞均有明显的抑制作用,抑瘤率分别为40.1%、26.92%,与对照组比较差异有统计学意义(P<0.05);4组中C-met均呈阳性表达,水蛭素高、低剂量组C-met的表达均下调,与阴性对照组比较差异有统计学意义(P<0.05)。结论水蛭素对小鼠喉癌细胞抑制作用显著。
【英文摘要】
Objective To investigate the inhibitory effect of hirudin on the laryngeal carcinoma tumor-bearing mouse.Methods 40 mice were randomly divided into 4 groups( n = 10),and planted with laryngeal cancer cells subcutaneous at the left armpit to set the tumor model. In positive control group,mice were intraperitoneal injected with fluorouracil( 20 mg/kg); in negative control group,mice received NS 0. 1 ml/( 10 g·d),in high-dose hirudin group,mice were accepted 2. 5 g/( kg·d). While low-dose hirudin group receive...
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