【摘要】 为了探讨丛枝真菌解除Pb胁迫下烟草氧化胁迫毒性的效应,采用盆栽试验,以烟草(Nicotiana tabacum L.)品种中烟100为材料,研究Pb胁迫下接种丛枝真菌对烟草根、叶Pb含量及叶片活性氧、丙二醛、丙酮醛含量及清除活性氧的抗氧化酶活性、酮醛转位酶活性和还原型谷胱甘肽含量的影响。结果表明,接种丛枝真菌后与同质量分数Pb胁迫相比,烟草根中Pb含量显著增加,叶片中Pb含量减少;叶片中过氧化氢含量和超氧阴离子产生速率、丙二醛含量均显著下降,超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶、过氧化物酶活性相应降低;酮醛转位酶I的活性升高,还原型谷胱甘肽含量增加。接种丛枝真菌可减少烟草叶片中的氧化胁迫,降低叶片Pb含量,提高烟叶质量。更多还原

【Abstract】 To study effects of inoculation arbuscular mycorrhizal fungi with tobacco on detoxification Pb stress,a pot experiment was carried out with tobacco( Nicotiana tabacum L.) and Glomus intraradice.The result showed that,under Pb stress,after inoculation Glomus intraradice with tobacco,Pb contentdecreased in leaves,but significant increase in roots. The H2 O2 content and production rate of superoxide aninwere decreased significantly,and the activity of SOD,CAT,APX,POD and MDA content decline accordingly. As glyoxalase I activity was enhanced and glutathione content increase in the leaves,the potent cytotoxic compound methylglyoxal decreased. The result indicated that inoculation arbuscular mycorrhizal fungi with tobacco could reduce oxidative stress and content of Pb in leaves,and consequently raise the quality of tobacco.更多还原