【摘要】 目的:探讨不同修复条件及修复液对食管鳞癌组织中ANO1的阳性表达程度的影响,筛选优势抗原修复方法。方法:对30例食管鳞癌组织的石蜡切片分别采用高压-枸橼酸缓冲液修复法、高压-EDTA缓冲液修复法、微波-枸橼酸缓冲液修复法及微波-EDTA缓冲液修复法进行修复,检测ANO1蛋白免疫组化染色效果。结果:食管鳞癌组织中ANO1蛋白经高压-枸橼酸缓冲液修复后,着色强度较其他修复方法明显增强,且阳性定位准确、对比度清晰,极大降低免疫组化背景着色。结论:在ANO1蛋白免疫组织化学染色中,高压-枸橼酸缓冲液修复法优于其他抗原修复方法。更多还原

【Abstract】 Objective: To explore the effect of different antigen repairing condition and repair liquid on degree of ANO1 positive expression in esophageal squamous cell carcinoma, thereby screening advantage antigen repair methods. Methods: The 30 cases of esophageal squamous cell carcinoma tissue paraffin section were respectively examined the immunohistochemical staining effects of ANO1 by high pressure-citrate buffer repair method, high pressure-EDTA buffer repair method, microwave-citrate buffer repair method and microwave-EDTA buffer repair method. Results: ANO1 protein in esophageal squamous cell carcinoma tissues was repaired by high pressure-citrate buffer, tinting strength enhanced obviously than other repair methods, and positive for accurate positioning, clear contrast, greatly reduce the background of immunohistochemical staining.Conclusion:The high pressure-citrate buffer repair method is superior to the other antigen repair methods in immunohistochemical staining of ANO1 protein.更多还原