【摘要】 目的探讨是否可通过使用钙敏感受体（CaSR）激动剂增强胱硫醚-γ-裂解酶（CSE）的表达促使内源性硫化氢（H₂S）的含量增加,从而抑制肺动脉平滑肌细胞（PASMC）的增殖、迁移。方法以人肺动脉平滑肌细胞（HPASMC）为研究对象,将样本设置为空白对照组（使用PBS液干预）、GdCl₃组（使用CaSR激动剂GdCl₃干预）、NPS2390组（使用CaSR抑制剂NPS2390干预）,在样本中加药孵育后,检测比较各组细胞增殖、迁移情况和细胞中Ca SR、CSE、H₂S的水平。结果三组细胞增殖、迁移情况和细胞中CaSR、CSE、H₂S的水平比较差异有统计学意义（P<0.05）。GdCl₃组细胞CaSR、CSE的表达和H2S的含量高于对照组,MTT实验吸光度（OD值）低于对照组,细胞迁移数少于对照组,差异有统计学意义（P<0.05）;NPS2390组细胞CaSR的表达和H2S的水平均低于对照组,MTT实验吸光度（OD值）高于对照组,细胞迁移数多于对照组,差异有统计学意义（P<0.05）。结论 Ca SR激动剂可通过增强CSE的表达增加H₂S的含量,进而抑制PASMC的增殖及迁移。更多还原

【Abstract】 Objective To investigate whether calcium-sensiing receptor（CaSR） agonist can increase the level of endogenous hydrogen sulfide（H₂S） by increasing the expression of cystathionine-γ-lyase（CSE）,and then inhibit the proliferation and migration of pulmonary artery smooth muscle cells.Methods Human pulmonary artery smooth muscle cells were divided into three groups:blank control group（PBS solution intervention）,GdCl₃ group（CaSR agonist GdCl₃ intervention）,NPS2390 group（Ca SR inhibitor NPS2390 intervention）.After incubated with drugs,the cell proliferation,cell migration and the expression of CaSR,CSE,H₂S were detected,and the above indexes were compared among the three groups.Results There was significant difference in the cell proliferation,cell migration and the expression of CaSR,CSE,H₂S among three groups（P < 0.05）,the expression of Ca SR,CSE and the level of H₂S in GdCl₃ group were significantly higher than those in control group（P < 0.05）,the OD value in GdCl₃ group was significantly lower than that in control group（P < 0.05）,the cell migration in GdCl₃ group was significantly less than that in control group（P < 0.05）;the expression of Ca SR and the level of H2 S in NPS2390 group were significantly lower than those in control group（P < 0.05）,the OD value in NPS2390 group was significantly higher than that in control group（P < 0.05）,the cell migration in NPS2390 group was significantly more than that in control group（P < 0.05）.Conclusions CaSR agonist can increase the level of H₂S by enhancing the expression of CSE,and inhibit the proliferation and migration of pulmonary artery smooth muscle cells.更多还原