【摘要】 目的:探讨单纯良性前列腺增生(BPH)与伴有前列腺炎的BPH患者前列腺组织中IL-17和IL-8表达差异。方法:收集行经尿道前列腺电切术的60例BPH患者标本。根据标本HE染色不同分为单纯BPH组23例和合并炎症的BPH组37例。对两组患者的临床资料进行分析比较;用免疫组化方法检测各标本IL-17和IL-8的表达情况,用荧光定量PCR和Western印迹检测各标本IL-17和IL-8 mRNA和蛋白的表达。结果:单纯BPH组和合并炎症的BPH组患者的国际前列腺症状评分(IPSS)[(21.6±3.7)分vs(29.1±6.2)分]、生活质量评分(Qo L)[(4.4±1.6)分vs(5.4±1.3)分]、最大尿流率(Qmax)[(8.2±1.8)ml/s vs(4.7±2.1)ml/s]、残余尿量(RUV)[(98.2±19.3)ml vs(198.6±15.5)ml]、前列腺体积[(49.8±16.5)ml vs(69.2±24.1)ml]、PSA[(2.8±0.8)μg/L vs(7.4±1.9)μg/L]和C反应蛋白(CRP)[(1.5±0.6)mg/L vs(5.1±2.0)mg/L],两组相比差异明显,有统计学意义(P<0.05);合并炎症的BPH组患者尿潴留的发生率[32.4%(12/37)]明显高于单纯BPH组[8.69%(2/23),P<0.05];IL-17 mRNA和IL-8 mRNA在合并炎症的BPH组前列腺组织中的表达量(0.303±0.076和0.536±0.059)均明显高于单纯BPH组(0.042±0.019和0.108±0.025,P<0.05);IL-17和IL-8蛋白在单纯BPH组和在合并炎症的BPH组患者前列腺组织中的蛋白表达(0.34±0.05 vs 0.88±0.10,0.43±0.04 vs1.07±0.08),两者相比差异显著,有统计学意义(P<0.05)。结论:IL-17和IL-8在合并炎症的BPH患者前列腺组织中的表达上调,其可能在BPH的发生发展中起重要作用。更多还原

【Abstract】 Objective:To investigate the expressions of interleukin-17(IL-17) and interleukin-8(IL-8) in benign prostatic hyperplasia(BPH) and BPH complicated with histological inflammation and their significance.Methods:According to the results of HE staining,we divided 60 cases of BPH treated by transurethral resection of the prostate(TURP) into a BPH group(n = 23) and a BPH with inflammation group(n = 37).We analyzed the clinical data of the patients and determined the mRNA and protein expressions of IL-17 and IL-8 by immunohistochemistry,real-time fluorescence quantitative PCR,and Western blot,respectively.Results:Compared with the BPH patients complicated with inflammation,the BPH group showed significantly lower International Prostate Symptom Score(IPSS)(29.1 ± 6.2 vs 21.6 ± 3.7),quality of life score(Qo L)(5.4 ± 1.3 vs 4.4 ± 1.6),postvoid residual urine volume(RUV)([198.6 ± 15.5] vs [98.2 ± 19.3] ml),prostate volume([69.2 ± 24.1] vs [49.8 ± 16.5] ml),PSA level([7.4 ± 1.9]vs [2.8 ± 0.8]μg/L) and serum c-reactive protein content(CRP)([5.1 ± 2.0]vs [1.5 ± 0.6]mg/L),but a higher maximum urine flow rate(Qmax)([4.7 ± 2.1]vs [8.2 ± 1.8]ml/s)(all P < 0.05).The former group had a significantly higher incidence rate of urinary retention than the latter(32.4% [12/37] vs 8.69% [2/23 ]),mRNA expressions of IL-17(0.303 ± 0.076 vs 0.042 ± 0.019) and IL-8(0.536 ± 0.059 vs 0.108 ± 0.025),and protein expressions of IL-17(0.88 ±0.10 vs 0.34 ± 0.05) and IL-8(1.07 ± 0.08 vs 0.43 ± 0.04)(all P < 0.05).Conclusion:The expressions of IL-17 and IL-8 are upregulated in the prostatic tissue of the BPH patients with inflammation,which may play a significant role in the development and progression of BPH.更多还原