[目的]从江苏如皋水稻田土样中分离到昆虫线虫一新株系N3(简称虫株N3),通过形态学和分子生物学特征对其进行了鉴定。[方法]利用光学显微镜和扫描电镜观察线虫的形态特征,对其形态特征进行测计并构建基于18S r DNA序列的系统发育树等,对虫株N3进行了系统分类鉴定。[结果]虫株N3主要形态特征是:食道基球退化;交合刺整体弧形弯曲,基部愈合弯曲;引带近腹部端,附着于交合刺基部;生殖乳突P3近泄殖腔,P2、P4远泄殖腔;双子宫。18S r DNA分子系统树表明:虫株N3与Diplogasteroides nasuensis最为相近,具有93.2%的同源性。虫株N3可以根据食道基球与交合刺基部的形态和引带附着位置加以区分。[结论]结合形态学和分子生物学特征鉴定,虫株N3为新种,命名为如皋似双胃线虫(Diplogasteroides rugaoensis n.sp.)。
【英文摘要】
[Objectives]An insectivorous nematode population N3( Hereinafter referred to as: N3) was collected from paddy field of Rugao,Jiangsu Province. Morphological and molecular biological methods were used for identification of the nematode population.[Methods]N3 was observed by scanning electron microscopy and light microscopy examination. Morphological characteristics of N3 were measured and calculated. Taxonomic status of N3 was analyzed by building phylogenetic trees based on 18 S r DNA sequences.[Results]The...
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