【摘要】 在水体的原位修复中,微生物的活性常受到温度、pH以及金属离子等的影响,因此强化及保持微生物的活性在实际工程应用中具有重要意义.以海藻酸钠为载体,对邻苯二甲酸酯降解菌M11进行固定化,并对比了固定化与非固定化邻苯二甲酸酯降解菌M11对邻苯二甲酸酯的降解能力.结果表明,同等条件下固定化M11对底物的降解性能明显优于游离态;以无机盐培养基作为其降解体系,反应温度控制在50℃,pH为8.0时,底物降解性能最优.固定化M11对底物邻苯二甲酸二丁酯的降解符合一阶动力学方程,且对底物浓度有一定的耐受范围;固定化M11的环境耐受性大大增强,反复利用性能良好.更多还原

【Abstract】 The activity of microorganism in water in-situ remediation is influenced by temperature,pH and the metal ion,so strengthen and maintain the activity of system has a great significance for the practical engineering application.The article immobilized the high efficient degradation bacteria M11 of the phthalic acid esters with the sodium alginate as a carrier and compared the degradation rate of the immobilized and free M11.The result revealed that the degradation rate of immobilized M11 are much better than free M11 under the same conditions.The immobilized M11 achieved the highest removal rate with 50 ℃,pH 8.0,a certain amount of the inorganic salt medium as its degradation system.The immobilized M11 had a certain tolerance range for the concentration of di-n-butyl phthalate(DBP),of which the degradation was in conformity with the first-order kinetics equation.Sodium alginate could protect the M11,which greatly enhanced the environment tolerance.In addition,immobilized M11 could be used repeatedly for a long time.更多还原