In the soil environment,glyphosate is apt to get adsorbed to soil particles,and react with organic matter and metal oxides in the soil,which may affect detection of glyphosate. Influences of humic acid content,heavy metal ion content and pH of the soil on detection of glyphosate were explored,using the sodium phosphate and trisodium citrate aqueous supersonic extraction method, hexane purification method, 9-fluorenylmethylchloroformate( FMOC-l) derivatization method and HPLC-FLD. It turned out that recovery...