【摘要】 首次从淡水育珠贝类池蝶蚌中得到多糖组分,探讨了相关提取工艺、理化特性以及体外抗氧化活性。Sevag工艺重复5次游离蛋白清除效果最佳,粗多糖经sevag纯化得率达到20.8%;通过离子交换树脂(DEAE-52)及葡聚糖凝胶柱层析(Sephacryl S-300)分离纯化得到池蝶蚌多糖(HSP-1),纯度达到97.5%;测定HSP-1的部分理化特性,测得其分子量为2 204Da,其单糖组成由64.8%的葡萄糖、17.4%的阿拉伯糖以及17.8%的葡糖醛酸组成;抗氧化活性测定结果表明,HSP-1对于DPPH、超氧自由基、羟自由基的最大清除率分别是62.16%,58.65%和45.21%。根据结果 HSP-1具有明显的抗氧化能力。更多还原

【Abstract】 The polysaccharides was purified from a freshwater pearl mussel Hyriopsis schlegelii,for the first time.This paper investigated the process of extraction,physicochemical properties and antioxidant activities in vitro of the polysaccharides.The optimal conditions of extraction are as follows:After using the Sevag methods to remove free proteins for 5times,the crude polysaccharides purity 20.8% was obtained.With Ion Exchange resin(DEAE-52)and Dextran Gel column Chromatography methods(Sephacryl S-300),the polysaccharides(HSP-1)were purified and the purification rate reached 97.5%.The physics and chemical characteristic property was determined and the molecular weight of HSP-1was 2204 D.And the monosaccharides was composed of glucose(64.8%),arabinose(17.4%)and glucuronic acid(17.8%).DPPH,superoxide anion radicals and hydroxyl radical scavenging of HSP-1were analyzed for antioxidant activity in vitro.The scavenge of polysaccharide,the DPPH,superoxide radical an hydroxyl radicals-scavenging activities was 62.16%,58.65%and 45.21%.These results suggested that polysaccharides extracted from Hyriopsis schlegelii show potent antioxidant activities.更多还原