【摘要】 目的:探讨PLGA神经导管联合ADSCs与自体神经组织碎屑修复大鼠坐骨神经缺损的修复效果。方法:32只SD大鼠平均分成4组,无菌条件下切断右侧的坐骨神经,制成1 0衄长的大鼠坐骨神经缺损模型,A组采用PLGA神经导管连接缺损神经进行修复,B组由内置自体神经组织碎屑的PLGA神经导管连接,C组由内置ADSCs与自体神经组织碎屑的PLGA神经导管连接,D组采用自体神经移植方式。12周后通过对再生神经桥接体的一般观察、HE染色、免疫荧光染色、甲苯胺蓝染色来评价神经的再生情况;通过肌电图、腓肠肌的HE和Masson染色来评价神经对靶器官的再支配情况。结果:术后12周,各组的神经导管均已降解,切断神经通过神经导管向两端生长。一般观察、肌电图、组织学观察结果均提示PLGA神经导管联合ADSCs与自体神经组织碎屑组C组的修复效果显著优于内置神经组织碎屑的PLGA神经导管组B组和空导管组A组的修复效果,但仍稍差于自体神经移植组D组。结论:PLGA神经导管联合ADSCs与自体神经组织可以比较有效地修复周围神经缺损,为周围神经缺损的修复提供了一种新的方法。更多还原

【Abstract】 Objective The aim of our research is to evaluate whether the combination of autologous minced nerve tissue and adipose derived stem cells(ADSCs) with biodegradable poly-lactic-co-glycolic-acid(PLGA) scaffolds can improve peripheral nerve regeneration effectively.Methods Thirty two Sprague-Dawley rats were divided equally into four groups,they are group A,B,C and D.Then the rats,sciatic nerves on the right sides were cut to create 10 mm defect models.After the 10 mm sciatic nerve defect models of the Sprague-Dawley rats were created,the sciatic nerves in group A,B,C and D were repaired with hollow PLGA nerve conduits,PLGA nerve conduits with autologous minced nerve tissue,PLGA nerve conduits combined with autologous minced nerve tissue and ADSCs,autologous nerve grafts respectively.After 12 weeks,analysis involved general observation,HE staining,immunofluorescence staining and toluidine blue staining of the regenerated nerves.Electromyogram,HE staining and Masson staining of the gastrocnemius muscle were performed to assess the targeted muscle reinnervation.Results Twelve weeks later,the PLGA nerve conduits were degraded completely and the regenerated nerves had connected the distal stumps and proximal stumps of the injured nerves.Besides,general observation,electromyogram and histomorphometric analysis indicated that the result of group C was significantly superior to group B and group A,but slightly inferior to group D.Conclusion The PLGA nerve conduits combined with autologous minced nerve tissue and ADSCs can repair peripheral nerve defects effectively and may serve as a promising strategy for peripheral nerve repair.更多还原