为进一步挖掘利用滨麦优异基因,并丰富小麦遗传种质资源,利用形态学、细胞遗传学、基因组原位杂交(Genomic in situ hybridization,GISH)、EST-STS分子标记、SSR分子标记等技术,对从八倍体小滨麦M842-16和硬粒小麦D4286杂交F_7代材料中筛选出的1个遗传稳定的小滨麦异代换系DM2411进行了鉴定。细胞遗传学观察表明,DM2411的染色体主要构型为2n=42=21Ⅱ,遗传稳定。根尖体细胞和花粉母细胞的原位杂交研究表明,DM2411含有1对滨麦Ns基因组。SSR分析表明,DM2411可能缺失了小麦2D染色体。EST分析表明,DM2411可能含有滨麦2Ns染色体。形态学调查表明,DM2411的株高极显著降低。
【英文摘要】
A wheat-L.mollis alien substitution line DM2411 was identified from the F_7 progenies of octoploid Tritileymus M842-16 and T.durum D4286 by using morphology,cytogenetics,genomic in situ hybridization(GISH),expressed sequence tag-sequence tagged site(EST-STS)and simple sequence repeat(SSR).Cytological studies demonstrated that DM2411 had a chromosome karyotype of 2n=42=21Ⅱ.GISH analysis indicated that DM2411 had a pair of Ns chromosomes fromL.mollis.Analysis with SSR and EST-STS markers showed that the wheat...