【摘要】 【目的】探讨海岛棉抗黄萎病材料的抗病机理,寻找可能的抗病基因。【方法】以高抗黄萎病海岛棉材料长312为试材,利用蛋白质双向凝胶电泳和质谱鉴定技术探讨棉花在大丽轮枝菌胁迫下蛋白质水平的变化。【结果】在接种大丽轮枝菌2 h后,长312叶片中有11个蛋白表达水平下调,15个蛋白表达水平上调;下调表达的蛋白主要是与光合作用及碳同化相关的一些酶类,推测大丽轮枝菌危害棉花叶片主要是破坏其光合系统;表达水平上调的蛋白主要是一些与光合作用、碳同化相关的酶类及苯醌还原酶、β-D-半乳糖苷酶、14-3-3f蛋白等抗病蛋白。【结论】推测海岛棉对黄萎病的防御机制发生在2个层面:一是被动防御,可能通过叶绿体ⅡA-B结合蛋白、核酮糖二磷酸羧化酶等下调蛋白的同工蛋白上调表达保持抗病海岛棉光合系统的稳定,通过组蛋白和14-3-3f蛋白的高表达保持抗病海岛棉植株细胞的稳定性;二是主动防御,可能通过高表达β-D-半乳糖苷酶和苯醌还原酶等参与海岛棉的抗病反应。更多还原

【Abstract】 [Objective] The purpose of this study is to analyze the resistance mechanism of island cotton under Verticillium wilt stress and to find possible resistance genes. [Method] The cotton resistance mechanism under the stress of Verticillium dahliae was studied by protein two-dimensional electrophoresis and mass spectrometry at the protein level. [Result] In the leaves, eleven proteins were down-regulated and 15 proteins were up-regulated after 2 hours of inoculation with Verticillium dahliae. The down-regulated proteins were mainly related to photosynthesis and carbon assimilation, and then we deduce that Verticillium dahliae is mainly broken cotton photosynthetic system to cause cotton susceptibility. The up-regulated proteins were mainly related to photosynthesis and benzoquinone reductase, beta-D-galactosidase, 14-3-3 f protein and other disease-resistant protein.[Conclusion] It is speculated that the defense mechanism of island cotton on Verticillium wilt may occur at two levels: one is passive defense, it is manifested that the isoproteins of the down-regulated proteins such as chloroplast II AB binding protein and ribulose diphosphate carboxylase are highly expressed to maintain the stability of the island cotton photosynthetic system, the histone and 14-3-3 f protein are highly expressed to keep the cell stability; the second is active defense, it is manifested that the high expressed β-D-galactosidase and benzoquinone reductase may be involved in the resistance of island cotton to Verticillium wilt.更多还原