【摘要】 【目的】MIKC~C是1类保守的转录因子家族,参与调控植物的开花时间和花器官发育。通过对MIKC~C家族进行全基因组学分析,为深入研究MIKC~C在棉花开花及花器官发育的分子调控机理提供基础。【方法】利用HMMER 3.0及pfam种子文件鉴定棉花全基因组MIKC~C基因,结合其表达量进行偏向表达及聚类分析。【结果】在陆地棉基因组中共鉴定发现100个MIKC~C基因,分为11个亚类。表达聚类分析显示,棉花MIKC~C基因的表达模式大致可分为4个不同的类群,说明这类基因在棉花进化中出现了功能分化。100个MIKC~C基因中,有12个基因具有miRNA靶位点。【结论】研究结果显示MIKC~C家族基因在棉花纤维中存在功能分化,而且可能受到miRNA的调控,这为进一步研究该家族基因的功能提供信息参考。更多还原

【Abstract】 [Objective] In plants, floral organ identity and the process of the transition from vegetative to reproductive development is regulated by MIKC~C gene family. Whole genome analysis is helpful to understand the evolutionary history of MIKC~C genes and provides the basis for an in-depth analysis of molecular mechanism of MIKC~C family in flowering and floral organ development in cotton. [Method] Whole genome identification of MIKC~C genes were carried by HMMER 3.0 and pfam gene bias expression and were clustered based on the RNA-seq data. [Result] We identified 100 MIKC~C genes and classified them into 11 subfamilies, according to their phylogenetic relationships to the Arabidopsis and Vitis vinifera MIKC~C genes. According to their expression profiles, MIKC~C genes were classified into four types. The MIKC~C genes of C/D and E subfamily were particularly highly expressed during cotton fiber elongation stage, indicating gene functional differentiation in cotton evolution. 12 of the 100 MIKC~C genes contains miRNA target sites, indicating that the flower development and the transition from vegetative to reproductive development of cotton may be regulated by miRNAs. [Conclusion] This study not only showed the functional differentiation of MIKC~C genes in cotton fiber development, but also found that MIKC~C family genes may be regulated by miRNAs in the growth and development of cotton, which provided the useful information for further functional studies of MIKC~C genes in cotton.更多还原