【摘要】 以毛竹实生苗为试验材料,研究不同浓度外源GA₃(0,0.1,0.5,1μmol·L^-1)对毛竹生长及CesA基因表达的影响。结果表明,与未经GA₃处理相比,施用外源GA₃后,毛竹实生苗茎节间和纤维细胞显著伸长,初生壁纤维素合成相关基因PeCesA2和PeCesA6相对表达量明显上调,次生壁相关基因PeCESA4和PeCESA4-1显著下调,傅里叶红外光谱分析（FTIR）,发现毛竹茎秆纤维素特征峰强度(1 060,1 160及1 373 cm^-1)随着GA₃浓度升高而逐渐增强,表明施用外源GA₃能够影响毛竹茎秆CesA基因表达,PeCesA2和PeCesA6的表达与外源GA₃促进纤维细胞伸长的过程存在一定联系。更多还原

【Abstract】 We studied the growth of stem and Ces A gene expression from the seedling in Phyllostachys edulis under the different concentration of exogenous GA₃( 0,0. 1,0. 5,1 μmol·L^-1). Compared with without GA₃ treatment,the internode and fiber cells of stem from the seedling elongated significantly,after treated by exogenous GA₃. The relative expression of primary wall-related PeCesA2 and PeCesA6 genes were significantly up-regulated,while that of secondary wall-related PeCesA4 and PeCesA4-1 genes were significantly downregulated. By Fourier transform infrared spectroscopy（ FTIR）,the absorption intensity of characteristic cellulose peak（ 1 060,1 160 and 1 373 cm-1） from the stems enhanced with the increase of GA₃ concentration.Signifying the expression of Ces A gene could be affected by the exogenous GA₃. The expression of PeCesA2 and PeCesA6 genes could be related to the process of fiber cells elongation.更多还原