【摘要】 目的:探讨接骨膏外敷给药对家兔骨折愈合过程中BMP7、CBFA1 mRNA表达的影响,分析其促进骨折愈合的作用机理。方法:建立30只家兔桡骨骨折模型,随机分为实验组和对照组,每组15只,实验组在其患处外敷接骨膏并用小夹板固定;对照组不使用药物,仅常规消毒后进行小夹板固定。分别于给药后第3、6、10周每组随机抽取5只,取患处组织标本,采用实时荧光定量PCR方法测定家兔骨组织中BMP7、CBFA1 mRNA的含量,对所得数据进行统计学分析。结果:实时荧光定量PCR检测结果显示:实验组家兔第3、10周末患处骨组织标本中BMP7、CBFA1 mRNA表达均高于对照组,差异有统计学意义(P<0.05)结论:接骨膏外敷给药可促进患处骨组织中BMP7、CBFA1 mRNA的表达,促进骨折愈合,缩短骨折的愈合时间。更多还原

【Abstract】 Objective: To discuss the influence of Jiegu Plaster on BMP-7 and CBFA1 mRNA expressions on rabbits fracture healing and analyze its mechanism of action to promote the fracture healing. Methods: Thirty rabbits with radius fracture were randomly divided into experimental and control groups,each group 15. The experimental group used Jiegu Plaster in bone lesion,fixed with a small splint. The control group was only fixed with small splint after routine disinfection. Respectively3,6 and 10 weeks after administration,randomly selecte 5 in each group to take out the affected tissue. Measure BMP7 and CBFA1 mRNA contents using real-time PCR for statistical analysis. Results: Real-time quantitative PCR results showed that the experimental group’ s BMP7 and CBFA1 mRNA expression at 3rd and 10 th weekend were higher than those of the control group and the difference was statistically significant( P < 0. 05). Conclusion: Jiegu Paste can promote BMP7 and CBFA1 mRNA expressions in bone tissue,promote healing and shorten the healing time of fractures.更多还原