【摘要】 目的:验证阳陵泉穴对艾灸治疗类风湿关节炎的抗炎增效作用,探讨少阳对骨之作用机制。方法:佐剂性关节炎大鼠40只,按治疗用穴不同,随机分为空白组;模型组;肾俞、足三里组;阳陵泉、肾俞、足三里组;太溪、肾俞、足三里组。各组造模后第7天,每日行小艾条温和灸,每组各穴单侧灸治5 min,每日1次,左右交替进行,6 d为1个疗程,共3个疗程。(1)ELISA法检测各组血清TNF-α、IL-6、NO水平;(2)RT-PCR法检测各组滑膜组织TNF-α mRNA表达。结果:艾灸阳陵泉、肾俞、足三里组血清TNF-α、IL-6、NO水平及滑膜组织TNF-α mRNA表达分别较肾俞、足三里组和太溪、肾俞、足三里组降低,差异有统计学意义(P<0.05)。结论:足少阳胆经阳陵泉穴对艾灸肾俞、足三里穴治疗佐剂性关节炎大鼠具有明确抗炎增效作用,提示少阳与骨存在密切生理联系。更多还原

【Abstract】 Objective: To explore the anti-inflammation of Yanglingquan( GB34) with the moxibustion treatment and the mechanism of Shaoyang meridian in treating bones and joints disease. Methods: Forty adjuvant arthritis rats modulated by complete freund’s adjuvant( CFA) were randomly divided into control group,model group,Shenshu( BL23) and Zusanli( ST36) group,Yanglingquan( GB34),Shenshu( BL23) and Zusanli( ST36) group,Taixi( KI3),Shenshu( BL23) and Zusanli( ST36) group. The treatment began seven days after modeling and moxibustion was applied on each acupoint by moxa cone 5 min for each acuponts,once a day and the acupoints were chosen alternately,6 days for 1 course,continued 3 courses. To detect TNF-α,IL-6 and NO contents in serum of rats by ELISA method. And the expression of TNF-α mRNA was tested by real time fluorescence quantitative PCR in synovial tissue in rats. Results: The inhibition effects on the TNF-α,IL-6 and NO in serum and TNF-α mRNA in the synovial tissues showed that Yanglingquan( GB34),Shenshu( BL23) Zusanli( ST36) group was the most significant effect group compared with Shenshu( BL23) and Zusanli( ST36) group and Taixi( KI3),Shenshu( BL23) and Zusanli( ST36) group.The contents of TNF-α,IL-6,NO in serum and TNF-α mRNA in the synovial tissues decreased in different degrees( P <0. 05). Conclusion: Moxibustion on Yanglingquan( GB34) shows a synergistic effect on anti-inflammation. It reveals the close relationship between Shaoyang and bones.更多还原