【摘要】 目的探讨抗CD137L单克隆抗体阻断CD137/CD137L信号通路对再生障碍性贫血(aplastic anemia,AA)T淋巴细胞亚群及单个核细胞体外诱生细胞因子水平的影响,及其在AA免疫治疗中的作用和意义。方法采用流式细胞术及ELISA方法,分析AA患者经抗CD137L单抗刺激前后骨髓T淋巴细胞亚群及单个核细胞诱生细胞因子IL-2、IFN-γ、IL-10的变化。结果 AA患者经抗CD137L单抗诱导后骨髓CD3+、CD4+、CD8+、CD25+T细胞比值显著降低(P<0.05),而对照组T细胞亚群几无影响。抗CD137L使再障患者骨髓单个核细胞IL-2、IFN-γ水平降低,但较正常对照组升高(P<0.05),使IL-10水平升高,但较正常对照组仍显著降低(P<0.05)。结论抗CD137L单抗可使AA患者T细胞亚群恢复或接近正常,使免疫状态得到改善,亦可使体外骨髓单个核细胞(BMMNC)中IL-2、IFN-γ水平下降,IL-10水平提高,具有重要意义。更多还原

【Abstract】 Objective To investigate the effect of blocking CD137/CD137 L signaling pathway on T lymphocyte subsets and cytokines induced by mononuclear cells in vitro, and their roles in aplastic anemia(AA) immunotherapy. Methods The changes of bone marrow T lymphocyte subsets and mononuclear cells inducing cytokines(IL-2, IFN-γ, and IL-10) in AA patients before and after stimulation with anti-CD137 L monoclonal antibody were analyzed by flow cytometry and ELISA. Results In patients with AA, the ratio of CD3+, CD4+, CD8+ and CD25+ T cells decreased significantly after the induction of anti CD137 L monoclonal antibody(P<0.05), while the T cell subsets in control group almost had no effect. Anti-CD137 L to patients with aplastic anemia bone marrow mononuclear cells of IL-2, IFN-γ levels decreased, but increased compared to normal control group(P<0.05), which elevated IL-10 levels, but still significantly lower than the normal control group(P<0.05). Conclusion Anti-CD137 L monoclonal antibody can make T cell subsets of AA patients recover or near normal, improve the immune state, and also decrease the level of IL-2 and IFN-γ in bone marrow mononuclear cell(BMMNC), and improve IL-10 level.更多还原