【摘要】 采用自动在线衍生-高效液相色谱法测定化妆品中游离甲醛的含量。样品经乙腈涡旋提取,以2,4-二硝基苯肼的乙腈-乙酸溶液为衍生液,在线衍生2min后,衍生物在Agilent C₁₈色谱柱上分离,以乙腈（65+35）溶液为流动相进行洗脱,用二极管阵列检测器测定,检测波长为355nm。甲醛线性范围为1.0²0.0mg·L^-1,测定下限（10S/N）为50mg·kg^-1。加标回收率在83.0%⁹8.4%之间,测定值的相对标准偏差（n=6）在3.4%⁷.8%之间。更多还原

【Abstract】 Free formaldehyde in cosmetic was determined by HPLC combined with automatic online derivatization.Free formaldehyde in cosmetic samples was extracted by acetonitrile and reacted efficiently with 2,4-dinitrophenylhydrazine in acetonitrile-acetic acid solution for 2 min.The formaldehyde derivative was separated on an Agilent C₁₈ chromatographic column with acetonitrile-water（65+35）as the mobile phase for elution,and then detected by DAD at the wavelength of 355 nm.The linear range of formaldehyde was 1.0-20.0mg·L^-1,with the lower limit of determination（10S/N）of 50mg·kg^-1.Recovery rates obtained by standard addition method were in the range of 83.0%-98.4% and RSDs（n=6）were in the range of 3.4%-7.8%.更多还原