【摘要】 目的:探讨肝细胞核因子1B(HNF1B)在前列腺癌中表达及其对肿瘤细胞生长和迁移的影响。方法:免疫组化检测HNF1B基因在患者前列腺癌组织中的表达;Western印迹法检测前列腺癌细胞系中HNF1B的表达水平。在DU-145细胞中过表达HNF1B,利用MTS检测HNF1B表达改变对细胞增殖的影响;克隆形成实验检测克隆情况;Transwell实验检测迁移情况。结果:前列腺癌组织中HNF1B蛋白表达低于癌旁组织(P<0.000 1),高转移、激素治疗抵抗的前列腺癌细胞HNF1B表达低于低转移前列腺癌细胞(P<0.05)。过表达HNF1B可抑制前列腺癌细胞增殖、迁移和克隆形成(P<0.05或P<0.01)。结论:HNF1B是前列腺癌抑癌基因,体外可通过抑制前列腺癌的增殖、迁移和生长发挥抑癌作用。更多还原

【Abstract】 Objective:To determine the role of hepatocyte nuclear factor 1B(HNF1B)in the proliferation and migration of prostate cancer cells.Methods:HNF1Bexpression in human prostate tissues was examined by IHC,and HNF1 B expression level in human prostate cells was examined by Western blotting.After over-expression of HNF1 Bin DU-145 cells,the cell proliferation was determined by MTS assay.Colony formation was examined by clone formation assay.Cell migration was examined by Transwell chamber assay.Results:HNF1Bexpressed relatively lower in tumor than that in benign tissues(P<0.000 1).HNF1 Bexpression in highly metastatic or hormonotherapy-resistant prostate cancer cells were lower than those in lowly metastatic prostate cancer cells(P<0.05).Over-expression of HNF1 Bin prostate cancer cells led to the suppression of cancer cell proliferation,migration,and colony formation(P<0.05 or P<0.01).Conclusions:As a tumor suppressor gene,HNF1 Binhibits prostate cancer cell proliferation,colony formation,and migration.更多还原