【摘要】 目的:概述基因检测对于个体化治疗的影响,通过国内外基因检测现状、基因检测主要方法及推荐进行检测的药物等方面阐述基因导向的个体化治疗的前景。方法:回顾1990年至2016年11月中国知网、维普、万方、Pub Med、SCI、FDA官网等数据库中有关基因检测方法、个体化治疗的文献,总结了基因检测对于个体化治疗的影响、国内外目前进展情况、基因检测方法及推荐进行检测的药物、目前存在的问题等方面的内容。结果:自精准医疗概念提出以来,国内外的基因检测都有了一定的进展;基因检测方法由最初的链终止法到现在的纳米孔单分子技术不仅增加荧光的信号强度及提高了仪器的灵敏度,更实现了高通量测序。结论:基因检测技术不仅增强了医疗工作者在基因组层面对疾病的认识,也使临床药学在传统服务模式的基础上,能够进一步分析遗传因素对药效的影响,使临床药学工作更加完整和可靠,实现针对性地药物选择和个体化治疗。更多还原

【Abstract】 Objective: Overview the effects of genetic testing for individual treatment, illustrate the prospects of gene oriented individualized therapy, main methods of genetic testing and recommended drugs needed genetic testing. Methods: This literature reviews all literatures that related method of genetic testing and individual therapy from database like CNKI, VIP data, Wan fang data, Pub Med, SCI, website of FDA official and other database during the year 1990 to 2016. It sums up the effects of genetic testing for individual treatment, the progress of genetic testing so far, genetic testing method and recommended drugs, challenges and so on. Results: Since the precise medicine concept has been put forward, situation of Domestic and foreign genetic testing have progress in a certain degree; genetic testing methods from the initial chain termination method to the current single-molecule nanopore technology, it is not only an increase in signal intensity of the fluorescence and improves the sensitivity of the instrument, but also achieves a high-throughput sequencing. Conclusion: Genetic testing technology not only enhances health professionals’ awareness of the disease at the genome level, but also enables clinical pharmacy to further analyze the impact of genetic factors on the efficacy based on the traditional model. By this way, clinical pharmacy’s work becomes more complete and reliable, achieving targeted selection of medication and individual therapy.更多还原