【摘要】 目的:观察SDF-1在大鼠颞下颌关节骨关节炎中的表达以及其与MMP-13、IL-1β和P38MAPK的相关性。方法:48只wistar大鼠随机分为对照组、模型组、高浓度抑制剂组和低浓度抑制剂组,采用关节腔注射碘乙酸钠溶液建立大鼠TMJOA模型。抑制剂组大鼠在建模后分别注射0.1μg/μL及1μg/μL SDF-1/CXCR4信号轴抑制剂AMD3100。后处死各组大鼠,取双侧颞下颌关节组织进行HE染色、番红固绿染色(S.O)以评价关节炎症水平;通过RT-PCR及免疫组化检测MMP-13、IL-1β的表达量;通过免疫组化染色检测p38MAPK。结果:对照组、高浓度抑制剂组、低浓度抑制剂组及模型组组织评分为:0.75±0.43、5±0.71、8±0.71、11.5±0.5。p-p38表达量为:(14.3±2.2)%、(41.5±2.2)%、(60.0±3.8)%、(74.9±3.5)%。IL-1β表达量为(12.8±1.0)%、(40.1±2.3)%、(64.2±4.4)%、(81.2±3.5)%;IL-1βm RNA为0.97±0.11、3.38±0.48、5.03±0.23、6.35±0.35。MMP-13表达量为(13.2±1.2)%、(38.2±2.3)%、(62.1±3.3)%、(80.9±3.0)%;MMP-13m RNA为0.96±0.18、2.54±0.41、5.24±0.45、6.31±0.35。对照组及模型组中SDF-1表达量为(26.1±3.2)%、(75.2±4.5)%;SDF-1m RNA为1.02±0.14、6.26±0.45。结果表明,随抑制剂剂量增加,关节炎症水平明显降低,MMP-13、IL-1β、p38MAPK表达量显著下调(P<0.05)。结论:SDF-1在颞下颌关节骨关节病的作用与促进MMP-13及IL-1β的表达和p38MAPK通路的激活相关。更多还原

【Abstract】 Objective: To investigate the effect of SDF-1 on expression of IL-1βMMP13 and P38 MAPK in rat temporomandibular joint osteoarthritis(TMJOA). Method:48 wistar rats were allocated to the control group,the pathologic model group or the antagonist groups. TMJOA was induce by injection of monosodium iodoacetate(MIA) into the upper compartment of bilateral TMJs of rats. SDF-1/CXCR4 axis antagonist AMD3100 were injected into the upper compartment of bilateral TMJs of rats in the antagonist groups after the pathologic model was established. Then all rats were sacrii ced. HE and Safranin O-fast green(S.O) staining were performed to evaluate the severity of TMJOA. The expressions of MMP-13,IL-1βwere detected by RT-PCR and Immunohistochemistry and the level of p38 MAPK were tested by Immunohistochemistry. Result: The pathological scores of control group,high or low antagonist concentration groups and pathologic model group were 0.75±0.43,5±0.71,8±0.71,11.5±0.5. The expression of p-p38 were(14.3±2.2)%,(41.5±2.2)%,(60.0±3.8)%,(74.9±3.5)%. The expression of IL-1β were(12.8±1.0)%,(40.1±2.3)%,(64.2±4.4)%,(81.2±3.5)%. The expression of IL-1βm RNA were 0.97±0.11,3.38±0.48,5.03±0.23,6.35±0.35. The expression of MMP-13 were(13.2±1.2)%,(38.2±2.3)%,(62.1±3.3)%,(80.9±3.0)%. The expression of MMP-13 m RNA were 0.96±0.18,2.54±0.41,5.24±0.45,6.31±0.35. The expression of SDF-1 in control group and pathologic model group were(26.1±3.2)%,(75.2±4.5)%. The expression of SDF-1m RNA were 1.02±0.14,6.26±0.45. Results shows with the increasing amount of antagonist applied, the severity of TMJOA and the expressions of MMP-13,IL-1βand p38 MAPK were significantly reduced.Conclusions: The pathological ef ect of SDF-1 on TMJOA is correlated with the increasing expression of MMP-13 and IL-1β and the activation of p38 MAPK.更多还原