【摘要】 目的观察血管平滑肌细胞(VSMCs)表型在增龄与高血压过程中的变化特点,以及增龄与高血压对miR-143/145表达的影响,从而探讨VSMCs表型转换和miR-143/145在增龄与高血压血管中的关系。方法研究对象为1、3、9、16月龄正常大鼠(WKY组)及原发性高血压大鼠(SHR组),分别对其血压、肠系膜动脉形态、VSMCs表型标志蛋白、miR-143/145的表达进行定量。结果血压和血管形态均随年龄和高血压发展有显著变化,VSMCs收缩表型标志蛋白α-actin和Calponin的表达在3月达到峰值后随年龄逐渐下降,合成表型标志蛋白OPN在SHR中随年龄上升,α-actin、Calponin和OPN在同龄的各年龄段WKY组和SHR组之间差异均具有显著性(P<0.05)。miR-143/145在发育过程中表达升高而随衰老减少,且在3月时WKY组表达显著高于SHR组(P<0.05)。结论高血压和增龄促进VSMCs由收缩表型向合成表型转换,miR-143/145的表达在高血压和增龄发展过程中被抑制,miR-143/145可能参与了高血压和增龄发生发展过程中VSMCs的表型转换过程。更多还原

【Abstract】 Aim This study was designed to explore the characteristics of VSMCs phenotype during aging and hypertension,and how miR-143/145 were influenced. Methods Mesenteric arteries from 1-,3-,9-,and 16-monthold WKY and SHR were isolated,artery histology were evaluated by staining with HE. VSMCs phenotype marker including α-actin,Calponin,and OPN were measured. miR-143/145 were quantified by real-time PCR. Results VSMCs contractile marker α-actin and Calponin expression both reached the peak at 3M and then decreased,while OPN increased with age in SHR with no obvious changes in WKY. The expressions of α-actin,Calponin,and OPN showed significant differences between SHR and the age-matched WKY. miR-143/145 firstly increased with age and then decreased in both WKY and SHR,and miR-143/145 in WKY-3M was significantly higher than the age-matched SHR. Conclusion Aging and hypertension accelerate the VSMCs phenotype switching in arterioles,promoting the synthesis phenotype;miR-143/145 is inversely regulated with contractile VSMCs and could play a role during aging hypertension.更多还原