【摘要】 [目的]化学感受蛋白(Chemosensory proteins,CSPs)广泛存在于昆虫的触角等化学感受器中,在昆虫的化学感受系统中发挥着重要的作用,为探究Amel-CSP3和Amel-CSP4在意大利蜜蜂Apis mellifera ligustica成年工蜂中的时空表达水平及功能。[方法]首先通过荧光定量PCR技术检测了Amel-CSP3和Amel-CSP4在哺育蜂和采集蜂的触角、头部、胸部、腹部和腿的表达水平;然后对不同日龄工蜂(1,6,12,18,28日龄)触角中Amel-CSP3和Amel-CSP4的表达情况进行检测。[结果]Amel-CSP3在哺育蜂和采集蜂触角均具有较高的表达水平,其在28日龄触角中的表达水平最高,显著的高于其他日龄(P<0.05);Amel-CSP4仅在工蜂触角表达,其在触角中表达水平随着工蜂日龄的增加而逐渐降低。[结论]Amel-CSP3和Amel-CSP4在意大利蜜蜂工蜂中的表达水平具有显著地时空特异性,这对探究Amel-CSP3和Amel-CSP4以及整个CSPs家族的功能具有一定的生物学意义。更多还原

【Abstract】 [Objectives] Chemosensory proteins are abundantly distributed in the antennae and other chemosensory organs of insects, and are believed to play important roles in the chemosensory system. This work aims to study the temporal and spatial expression levels, and explore the functions of, two chemosensory proteins, Amel-CSP3 and Amel-CSP4, in adult Apis mellifera ligustica workers. [Methods] Expression levels of Amel-CSP3 and Amel-CSP4 in different organs of nurses and foragers, and in the antennae of 1, 6 12, 18 and 28 day-old workers, were quantified with RT-qPCR. [Results] Amel-CSP3 was more highly expressed in the antennae of both nurses and foragers, and its expression level in the 28 day-old workers was significantly higher than that in younger workers(P<0.05). Amel-CSP4 was only expressed in workers’ antennae, where its expression decreased with age. [Conclusion] The expression of Amel-CSP3 and Amel-CSP4 had a high degree of spatiotemporal specificity in adult Apis mellifera ligustica workers. This finding provides important information for exploring the function of Amel-CSP3, Amel-CSP4, and other chemosensory proteins, in A. m. ligustica.更多还原