【摘要】 目的探索P120-连环蛋白(P120ctn)在口腔鳞状细胞癌(OSCC)上皮-间质转化(EMT)中的作用。方法采用质粒pGFP-V-RS-P120ctn shRNA转染人舌鳞癌细胞(TSCCA),采用实时荧光定量聚合酶链反应、Western blot检测细胞中P120ctn、E-钙黏蛋白(E-cad)、N-钙黏蛋白(N-cad)和波形蛋白(Vim)的mRNA和蛋白表达的变化,通过Transwell细胞侵袭及细胞迁移试验检测转染前、后细胞迁移和侵袭能力的变化。结果用质粒pGFP-V-RS-P120ctn shRNA转染TSCCA后发现,随着P120ctn表达的明显降低,E-cad的mRNA和蛋白表达水平明显降低,N-cad和Vim的mRNA和蛋白表达水平明显升高,细胞迁移和侵袭能力明显提高,差异均有统计学意义(P<0.05)。结论在OSCC中P120ctn可能通过调控EMT标记物E-cad的表达参与EMT的发生,调节肿瘤的侵袭和转移。更多还原

【Abstract】 Objective To investigate the role of P120-catenin(P120ctn)in the process of the epithelial-mesenchymal transition(EMT)of oral squamous-cell cancer(OSCC).Methods Plasmid pGFP-V-RS-P120 ctn shRNA was used to transfect TSCCA cells.Real-time fluorescent quantitative PCR and Western blot were adopted to test mRNA and protein expressions of P120 ctn,E-cadherin(E-cad),N-cadherin(N-cad)and Vimentin(Vim),and Transwell cell invasion and cell migration assay were used to test the invasion and migration capacity before and after the transfection.Results After plasmid pGFP-V-RS-P120 ctn shRNA was used to transfect TSCCA cells,we found that as the expression of P120 ctn significantly decreased,mRNA and protein expression of E-cad decreased significantly,mRNA and protein expression of N-cad and Vim increased significantly,and cell migration and invasion capacity increased significantly(P<0.05).Conclusion In OSCC cells,P120 ctn may be involved in EMT and the metastasis and invasion regulation by adjusting EMT biomarker E-cad expression.更多还原