【摘要】 采用SSR分子标记对17个水稻光温敏不育系进行遗传多样性分析。结果表明,在水稻基因组中较均匀分布的168个SSR位点中91个SSR位点具有多态性,多态性频率为54.17%。91个SSR标记共检测出229个等位基因,每个SSR位点检测到2~4个,平均2.517个等位基因,平均多态性信息含量指数(PIC)为0.412。根据17份光温敏不育系的遗传相似系数矩阵作出树状图,聚类分析结果表明17个不育系遗传相似系数变幅为0.59~0.93;以遗传相似系数0.61为阀值,可将供试不育系分为4个群,聚类分析结果与亲缘系谱分析结果基本吻合。更多还原

【Abstract】 The genetic diversities of 17 rice PTGMS lines were analyzed by adopting 168 pairs of SSR markers which were evenly distributed on the 12 chromosomes of rice. Among these 168 pairs of SSR markers,91 pairs of SSR markers showed a polymorphism with the polymorphic frequency of 54.17%. A total of 229 alleles were detected with these 91 polymorphic SSR markers,and the number of alleles per SSR locus ranged from 2 to 4 with an average of 2. 517. The average polymorphic information content index was 0.412. According to the genetic similarity coefficient matrix of the tested 17 rice PTGMS lines,a dendrogram was constructed by UPMGA,and a clustering analysis was conducted,the results showed that the range of genetic similarity coefficient of 17 rice PTGMS lines was 0.59 ~ 0.93. When genetic similarity coefficient 0.61 was used as a threshold,the tested rice PTGMS lines could be divided into four groups,which was basically consistent with their pedigree relationship.更多还原