【摘要】 目的研究江西省HIV/AIDS(艾滋病感染者/艾滋病病人)人群趋化性细胞因子配体蛋白CCL3L1(CC chemokine ligand 3-like-1)的拷贝数及其与HIV病毒(艾滋病病毒)易感性及艾滋病进展的关系。方法收集192例HIV/AIDS和131例阳性者配偶阴性血样标本,提取基因组DNA,应用PRT(the Paralogue Ratio Test)法检测其CCL3L1基因拷贝数,计算江西省人群CCL3L1基因拷贝数中位数,用SPPS统计软件分析CCL3L1拷贝数与HIV感染的关系。结果江西人群CCL3L1基因拷贝数中位数为2;HIV慢性感染者与健康人无显著性差异(P>0.05);第一次CD4淋巴细胞数与基因拷贝数的变化有显著相关(P<0.05)。结论江西人群的CCL3L1基因拷贝数与HIV易感性无相关性,与感染进程有一定相关性。更多还原

【Abstract】 Objective To study the chemokine CCL3L1 gene copy number of people in Jiangxi province and its relationship with HIV-1 infection. Methods Blood samples collected from the 192 cases of HIV/AIDS and 131 cases of HIV positive spouse who are HIV negative were used for the extraction of genomic DNA. The method of PRT(the Paralogue Ratio Test) was used to detect gene copy number of its CCL3L1,with that we calculated the median of CCL3L1 copy number in Jiangxi province. We investigated the relationship of CCL3L1 gene copy number with HIV by SPPS statistical software. Results The median of CCL3L1 copy number in Jiangxi province is two,and there is no statistical significant differences(P>0.05) between the CCL3L1 copy number of HIV infected patients and that of healthy people. The first CD4 lymphocytes count after diagnosed is significantly correlated(P <0.05) with CCL3L1 gene copy number. Conclusion The CCL3L1 gene copy numbers of people might not associate with the susceptibility to HIV but correlate with the progress of HIV/AIDS in Jiangxi.更多还原