【摘要】 目的调查天津某地小区生活饮用水中非苛养耐药菌的污染状况。方法采用R2A琼脂对自来水进行细菌分离,利用16S rRNA基因测序法对细菌分离株进行种属鉴定。同时采用Kirby-Bauer纸片扩散法(K-B纸片法)进行细菌的抗生素敏感性试验。结果在分离的39株非苛养菌中,耐药菌占79.49%,耐药细菌涉及肠球菌属、葡萄球菌属、不动杆菌属、假单胞菌属。其中,仅对一种抗生素耐药的细菌占28.21%,而多药耐药菌则占51.28%。耐药菌对复方新诺明的耐药最为普遍,耐药率为53.85%;对磺胺甲噁唑的耐药次之,占28.21%。结论该小区生活饮用水中的非苛养耐药菌污染较为严重,应加强关注其潜在的人群风险。更多还原

【Abstract】 Objective To study the non-fastidous antibiotic-resistance bacteria pollution in tap water from a neighborhood in Tianjin. Methods Tap water samples were collected and bacteria were isolated by R2 A agar,and 16 S rRNA gene sequence for the identification of bacteria isolates was used. Antibiotic susceptibility testing was performed using the Kirby-Bauer disc diffusion method. Results Among the 39 non-fastidous bacteria isolates,the resistance rate of antibioticresistance bacteria was 79. 49%,including Enterococcus,Staphylococcus,Acinetobacter and Pseudomonas. Some bacteria( 28. 21%) showed resistance against only one antibiotic. The others( 51. 28%) were multiple resistant bacteria.Trimethoprim/sulfamethoxazole resistance was most prevalent( 53. 85%),and sulfamethoxazole resistance was also widely distributed( 28. 21%). Conclusion Tap water from this neighborhood is heavily polluted by non-fastidous antibioticresistantance bacteria,which deserves more attention.更多还原