【摘要】 背景:有研究提示5-羟色胺系统的功能障碍与强迫症(obsessive-compulsive disorder,OCD)有关。目标:本研究拟定量OCD患者的外周SLC6A4基因表达,以探讨SLC6A4和OCD之间的关系。方法:受试为50例首发OCD患者和60例年龄和性别匹配的健康对照,通过实时定量逆转录聚合酶链反应(real-time quantitative reverse transcription polymerase chain reaction,RT-PCR)定量外周白细胞SLC6A4基因表达。采用Yale-Brown Obsessive Compulsive Scale(Y-BOCS)评估OCD的严重性和亚型。结果:以甘油醛-3-磷酸脱氢酶(GAPDH)标准化定量外周SLC6A4基因表达量,在OCD组和健康对照组(z=0.79,p=0.428)之间没有显著差异。男性OCD患者显示外周SLC6A4低基因表达的倾向(z=-1.66,p=0.096)。研究也未能发现SLC6A4基因表达量与OCD的严重性和亚型之间有显著相关性。结论:SLC6A4基因表达量与OCD的严重程度和亚型之间没有相关性,但男性OCD患者呈外周SLC6A4低基因表达倾向。本研究表明,中国汉族人群的外周SLC6A4基因表达可能并非有效的生物标记物。更多还原

【Abstract】 Background: Serotonergic system dysfunction has been implicated in obsessive-compulsive disorder(OCD). This study examined peripheral SLC6A4 gene expression in OCD patients and healthy controls to explore the relationship between SLC6A4 and OCD. Methods: Participants included 50 first episode OCD patients and 60 age and gender-matched healthy controls. Relative SLC6A4 gene expression were examined by real-time quantitative reverse transcription polymerase chain reaction(RT-PCR) in peripheral leukocytes of all the subjects. The Yale-Brown Obsessive Compulsive Scale(Y-BOCS) was used to assess the severity and subtype of OCD. Results: SLC6A4 gene expression, normalized by glyceraldehyde-3-phosphate dehydrogenase(GAPDH), were not significantly different between the OCD patients and healthy controls(z=-0.79, p=0.428). Male OCD patients showed a tendency of low gene expression of SLC6A4 in peripheral blood(z=-1.66, p=0.096). We did not find a significant correlation between SLC6A4 expression and the severity and subtype of OCD. Conclusion: There is no correlation between SLC6A4 expression levels and the severity and subtype of OCD, but male OCD patients showed a tendency of low gene expression of SLC6A4 in peripheral blood. These results suggest that gene expression of SLC6A4 in peripheral blood may not be a useful biomarker of OCD in the Han Chinese population.更多还原