One hundred and twenty cultivars or advanced lines were selected from different wheat growing regions,and were milled into flour. The real content of SDS-unextractable polymeric protein(UPP) in the flour extracted by phosphate buffer was quantified by high performance liquid chromatograph method. At the same time,the spectrum data of UPP in the phosphate buffer was collected based on near infrared reflectance spectroscopy technique. The calibration model was created based on Unscrambler software and partial...