【摘要】 目的:探讨槲皮素能否减轻紫外线照射诱导的人精子氧化损伤。方法:通过收集10例健康志愿者精液,密度梯度法离心分离精子,利用紫外线照射90 s诱导精子氧化损伤。实验组添加50μmol/L槲皮素培养,采用计算机辅助精液分析系统(CASA)检测精子活动力,吖啶橙(AO)试验检测DNA损伤,流式细胞术检测活性氧(ROS)以及TUNEL法检测精子凋亡。结果:CASA显示各组精子活动力没有明显改变,AO试验表明实验组精子DNA损伤减轻,流式细胞术结果显示实验组精子内ROS含量下降,同时凋亡也相应减少。结论:槲皮素通过降低ROS,减轻紫外线照射诱导的人精子氧化损伤,从而起到保护作用。更多还原

【Abstract】 Objective:To evaluate whether quercetin could reduce oxidative damage in human sperm induced by ultraviolet radiatiown b irradiation.Methods:Ten semen samples from healthy volunteers were collected,centrifuged and isolated by density gradient centrifugation method,and induced oxidative damage by using 90 seconds of ultraviolet radiation.Sperms were cultured 1 h in vitro after quercetin(50 umol/L) was added,then collected to detect sperm viability and motility by computer-assisted semen analysis system(CASA).In the meantime,DNA damage of sperms was detected by acridine orange test,reactive oxygen species(ROS) content and sperm apoptosis were detected by flow cytometry.Results:UVB irradiation led to an increase in sperm DNA damage,ROS production and apoptosis,and quercetin could reverse it,but had no effect on sperm motility.Conclusion:Quercetin plays a protective role in human sperm oxidative damage induced by UVB irradiation mainly by reducing the ROS content.更多还原