【摘要】 目的探讨miR-372在卵巢癌细胞上皮间质转化(EMT)、侵袭迁徙中的作用及相关的机制。方法利用Lipofectamine2000瞬时转染miR-372 mimics于OVCAR3和A2780细胞中,并通过RT-PCR法检测在转染前后OVCAR3和A2780细胞中miR-372的表达,Transwell及细胞划痕实验检测OVCAR3和A2780细胞的侵袭迁徙能力,蛋白印迹法检测EMT相关蛋白N-钙黏蛋白和a-SMA蛋白的表达水平。结果转染miR-372 mimics后OVCAR3和A2780细胞的miR-372相对表达量显著升高(P<0.05),细胞的侵袭迁徙能力显著降低(P<0.05);Western Blotting结果表明转染miR-372 mimics后OVCAR3和A2780细胞的EMT相关间质蛋白N-钙黏蛋白和a-SMA蛋白的表达水平显著降低。结论miR-372过表达抑制卵巢癌细胞的体外侵袭及迁徙,并通过抑制N-钙黏蛋白和a-SMA蛋白水平的表达来抑制卵巢癌细胞的EMT过程。更多还原

【Abstract】 Objective and To investigate the role and mechanism of miR-372 mimics in epithelial-mesenchymal transfected transition(EMT),and invasion by migration of ovarian the cancer cells.Methods miR-372 detected were into and OVCAR3cell A2780 were cells used Lipofectamine2000,expression of miR-372 was by RT-PCR.Transwell scratch a-SMA test to detect the invasion Western and migration ability.The expression of EMT-related proteins N-cadherin and protein was detected by blotting.Results The miR-372 expression level in OVCAR3 and A2780 cells was mimics(P<0.05).The invasion and migration ability of OVCAR3 and significantly A2780 increased were after transfection of miR-372 after transfection(P<0.05).Western blotting results showed that cells significantly decreased miR-372 mimics miR-372 overexpression reduced the expression level of EMT-related interstitial proteins N-cadherin and a-SMA EMT proteins.inhibiting Conclusion the miR-372 overexpression inhibits ovarian cancer cell invasion and migration,and inhibits via expression of N-cadherin and a-SMA protein in ovarian cancer cells.更多还原