【摘要】 目的探讨腹腔注射Sigma-1受体激动剂PRE-084对创伤后应激障碍(PTSD)大鼠额叶神经元损伤的影响。方法利用单程长时应激(SPS)制备PTSD大鼠模型,将PTSD大鼠分为4组:vehicle、PRE-084、SPS+vehicle和SPS+PRE-084组;连续注射PRE-084 7d后,取材各组大鼠额叶,利用免疫荧光染色检测各组大鼠额叶Neu N表达情况,免疫荧光染色、Western blot检测p-ERK蛋白表达情况。结果免疫荧光染色结果显示,PTSD大鼠额叶神经元数量降低,腹腔注射PRE-084可以显著改善PTSD引起的神经元损伤;Western blot结果显示,PTSD大鼠额叶p-ERK表达水平降低,PRE-084可以显著升高p-ERK蛋白水平。结论腹腔注射Sigma-1受体激动剂PRE-084改善PTSD大鼠额叶神经元损伤可能与上调ERK信号通路相关。更多还原

【Abstract】 Objective To investigate the effect of Sigma-1 receptor agonist(PRE-084)on damaged neurons in prefrontal cortex of post-traumatic stress disorder(PTSD) rats. Methods The PTSD model was established by single prolonged stress paradigm(SPS). The animals were divided into 4 groups: vehicle, PRE-084, SPS+ vehicle and SPS+ PRE-084 group. The expression of Neu N in prefrontal cortex of rats was detected by immunofluorescence staining. The expression of p-ERK protein was detected by immunofluorescence staining and Western blot respectively. Results The percentage of Neu N positive cells in prefrontal cortex of SPS rats was decreased, and PRE-084 upregulated the number of Neu N positive cells. The expression level of p-ERK in prefrontal cortex was decreased in SPS rats than in normal rats, but increased in PRE-084 injection group than in SPS rats. Conclusion The improvement of the activity of neurons in prefrontal cortex of SPS rats by Sigma-1 receptor agonist(PRE-084) might be related to the upregulation of ERK pathway.更多还原