【摘要】 目的探讨携带血管内皮生长因子165(vascular endothelial growth factor 165,VEGF165)基因的腺病毒(Ad-VEGF165)转染至心肌样细胞对其表达VEGF165及增殖活性的影响。方法提取、培养、鉴定骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs);体外诱导BMSCs为心肌样细胞。构建携带VEGF165基因的腺病毒载体并转染至心肌样细胞;实验分为3组:Ad-VEGF165转染心肌样细胞作为实验组;未携带VEGF165基因的腺病毒转染心肌样细胞作为空载体组;未处理的心肌样细胞作为空白对照组。在1周内检测各组VEGF165的表达及心肌样细胞的增殖活性。结果 VEGF165在实验组可正常表达,与空载体组、对照组相比差异具有统计学意义(P<0.05);心肌样细胞增殖活性在实验组中最佳,显著优于空载体组和对照组(P<0.05)。结论成功将Ad-VEGF165转染至心肌样细胞,且表达的VEGF165可增强心肌样细胞的增殖活性。更多还原

【Abstract】 Objective Vascular endothelial growth factor 165(VEGF165) gene adenovirus was transfected into myocardial cells to investigate the expression of VEGF165 and proliferation activity of influence.Methods Bone marrow mesenchymal stem cells(BMSCs) were extracted,cultured and identified.The BMSCs were induced myocardial cells in vitro.The adenovirus vector carrying VEGF165 gene was constructed and transfected into myocardial cells.The experiment was divided into three groups:Ad-VEGF165 was transfected into myocardial cells as the experimental group.No carrying VEGF165 gene adenovirus was transfected into myocardial cells as empty carrier group.Untreated myocardial cells were as blank control group.Within 1 week between groups the expression of VEGF165 and myocardial cells proliferation activity was detected.Results The experiments showed that the experimental group VEGF165 could be expressed,and compare with the empty vector group and the control group with statistical significance(P < 0.05).The myocardial cells in the experimental group had the best proliferation activity,and significantly better than an empty vector group and the control group(P < 0.05).Conclusion The Ad-VEGF165 was transfected into myocardial cells,and VEGF165 could enhance the activity of cell proliferation.更多还原