【摘要】 采用热水抽提和乙醇沉淀的方法对红毛藻(Bangia fusco-purpurea)粗多糖组分进行提取分离,利用Sephadex G75凝胶柱层析和DEAE Cellulose 52阴离子交换柱层析纯化后,分析其对血管紧张素转换酶(angiotensin converting enzyme,ACE)活性的抑制作用,并利用高效液相色谱和离子色谱等方法,分析活性片段的组成特征,利用酶动力学研究红毛藻多糖活性片段对ACE活力的抑制类型。结果表明,经提取和分离纯化后,得到3个多糖片段P1、P2和P3。酶活性分析结果表明,多糖组分P1对ACE活性有显著的抑制作用,并且抑制作用呈现多糖浓度依赖性,其IC50为0.34 g/L。利用高效液相色谱法和离子色谱法对P1的单糖组分和硫酸基团含量进行分析,结果表明,P1主要由阿拉伯糖(88.07%)、葡萄糖(4.55%)和糖醛酸(6.67%)组成,硫酸根质量分数为0.02%。酶促动力学和Lineweaver-Burk作图分析结果表明,P1对ACE的抑制类型为可逆非竞争性抑制。更多还原

【Abstract】 The crude polysaccharides were extracted from Bangia fusco-purpurea by soaking in hot water and alcohol precipitation. Sephadex G75 gel filtration chromatography and DEAE ion exchange chromatography were used for the purification. The inhibitory activity and type on angiotensin converting enzyme( ACE) were analyzed by enzyme kinetic assay. The composition of polysaccharide fraction was investigated by HPLC and ion chromatography. The main results were as follows: the enzyme activity analysis demonstrated that P1 significantly inhibited the activities of ACE in a concentration-dependent manner,and the IC50 values of P1 against ACE were estimated to be 0. 34 g/L. The sugar composition analysis revealed that P1 fraction mainly consisted of arabinose( 88. 07%) together with a small amount of uronic acid( 6. 67%), glucose( 4. 55%) and sulfate groups( 0. 02 %). Furthermore,the results of the kinetic analysis demonstrated that P1 inhibited ACE through the reversible inhibition and its inhibition type followed non-competitive inhibition.更多还原