【摘要】 目的探讨小泛素化修饰增强子(RSUME)、核转录因子-κB抑制因子-α(IκB-α)及核转录因子-κB 1(NF-κB1)在小鼠垂体腺瘤中表达及其细胞凋亡的影响。方法用RSUME小干扰RNA(siRNA)转染At T-20细胞后,用蛋白印迹及实时荧光定量PCR检测RSUME、NF-κB1和IκB-α蛋白的表达及mRNA的表达,流式细胞计量术检测细胞凋亡。结果在蛋白水平,RSUME-siRNA转染后RSUME及IκB-α表达下调(P<0.05),NF-κB1则上调(P<0.05);在mRNA水平,转染后RSUME mRNA表达水平明显低于转染前(P<0.05),而IκB-α及NF-κB1 mRNA水平无明显变化;转染后细胞凋亡率明显升高。结论 RSUME可以通过NF-κB1促进小鼠At T-20垂体腺瘤细胞的凋亡。更多还原

【Abstract】 Objective To explore the expression and influence of RWD structure small ubiquitin modified enhancer(RSUME) and inhibitor of nuclear factor kappa B-alpha(IκB-α) and nuclear factor kappa B-1(NF-κB1)in pituitary adenomas of mice.Methods Real-time fluorescent quantitative PCR(q-PCR) was used to detect the level RSUME,IκB-α,NF-κB1 mRNA and Western blot was used to detect RSUME and the nucleoprotein of IκB-α,NF-κB1 changes in the level of protein,flow cytometry was used to detect cell apoptosis.Results In protein level,RSUME and IκB-α expression reduced(P < 0.05),but NF-κB1 raised after transfection(P <0.05); The level of RSUME mRNA was obviously lower after transfection than before(P < 0.05).The level of IκB-α and NF-κB1 mRNA was not significantly changed; Flow cytometry confirmed cell apoptosis rate increased significantly after transfection.Conclusions RSUME can promote apoptosis of pituitary adenoma cells in mice by NF-κB.更多还原