【摘要】 为探讨益生性枯草芽孢杆菌对肠上皮细胞黏附及对嗜水气单胞菌的抑制作用,以草鱼肠上皮细胞为研究对象,检测了枯草芽孢杆菌对肠上皮细胞的黏附率、对嗜水气单胞菌的黏附抑制率和对各种生理生化指标的影响。结果显示:枯草芽孢杆菌对草鱼肠上皮细胞形态、细胞中四甲基偶氮唑盐(MTT)OD值、乳酸脱氢酶(LDH)、碱性磷酸酶(AKP)和谷草转氨酶(GOT)活性等各种生理指标无显著影响,但在孵育3h和6h后分别显著降低了Na+,K+-ATP酶和谷丙转氨酶(GPT)的活性;与嗜水气单胞菌孵育3h后,肠上皮细胞由椭圆变成不规则形态,培养液的死细胞增多,并且在多数时间点上显著降低了MTT OD值以及谷草转氨酶、Na+,K+-ATP酶和谷丙转氨酶的活性,细胞中MTT OD值和细胞上清中乳酸脱氢酶的活性显著提高(P<0.05);枯草芽孢杆菌和嗜水气单胞菌共孵育对肠上皮细胞形态及各种生理指标的影响明显小于嗜水气单胞菌组;黏附抑制实验显示枯草芽孢杆菌可以显著降低嗜水气单胞菌对肠上皮细胞的黏附率。提示枯草芽孢杆菌可以抑制嗜水气单胞菌对草鱼肠上皮细胞的黏附,并减轻嗜水气单胞菌对细胞的损伤。更多还原

【Abstract】 To investigate the adherence of Bacillus subtilis and its inhibitory of Aeromonas hydrophila,the adherence rate of B.subtilis,its inhibition rate of A.hydrophila,and various physiological and biochemical indexes were detected in the intestinal epithelial cells of grass carp.The results showed that after treatment of B.subtilis alone,cell morphology,methyl thiazolyl tetrazolium optical density(MTT OD)value,lactate dehydrogenase(LDH),alkaline phosphatase(AKP)and glutamicoxalacetic transaminase(GOT)activity were unsignificantly changed(P>0.05),but the activities of Na+,K+-ATPase and glutamic-pyruvic transaminase(GPT)significantly increased(P <0.05)at 3h and 6h,respectively.The cell shape was obviously changed by A.hydrophilafrom ellipse to irregular shape and the number of dead cells in the culture medium increased.Meanwhile,A.hydrophila could significantly increase MTT OD value and LDH activity in the culture medium,and significantly decrease the activities of Na+,K+-ATPase,GOP and GPT in the cells(P <0.05).The adherence experiment showed that B.subtilis could significantly reduce the adhesion of A.hydrophilato grass carp intestinal cells.In brief,adhesion and damage of A.hydrophila to intestinal epithelial cells were inhibited by B.subtilis.更多还原