【摘要】 目的从巴沙鱼皮中提取胶原蛋白并分析其结构类型及氨基酸组成,同时研究物理、化学交联法对其变性温度的影响。方法采用酸提法和酶提法提取巴沙鱼皮中的胶原蛋白,并用SDS-PAGE凝胶电泳、FTIR和HPLC分析胶原蛋白的结构类型和氨基酸组成。通过测定黏度值研究物理、化学交联法对其变性温度的影响。结果酸提法提取胶原蛋白的提取率为58.2%,酶提法的提取率为22.0%;巴沙鱼皮鱼胶原蛋白为I型胶原蛋白;经120℃热交联后,巴沙鱼皮胶原蛋白的变性温度从34.2℃下降至28.0℃,经四甲基乙二胺(EDC)交联后,其变性温度上升至35.8℃。结论巴沙鱼皮富含I型胶原蛋白,酸提法比酶提法提取效率高,采用化学交联法可提高其变性温度,因而巴沙鱼皮可作为胶原蛋白的重要来源,可用于制备多种生物医用材料,具广泛的应用前景。更多还原

【Abstract】 Objective To study the extraction,the collagen type and the amino acid composition of collagen fromPangasisus haniltoa skin,and investigate the effects of denaturation temperature treated by physical and chemical crosslinking methods.Methods Collagen of Pangasisus haniltoa skin was extracted by acid-soluble and pepsin-soluble methods.Collagen structure type was analyzed and confirmed by SDS-PAGE gel electrophoresis,FTIR and HPLC.The denaturation temperature was studied by detecting the viscosity.Results The extraction rates of collagen fromPangasisus haniltoa skin by acid-soluble and pepsin-soluble methods was 58.2% and 22.0%,respectively.The collagen from Pangasisus haniltoaskin was type I collagen.The denaturation temperature declined to 28.0℃from 34.2℃ when the collagen was tried by 120℃crosslinking method and it was raised to 35.8℃ when the collagen was tried by EDC crosslinking method.Conclusion Pangasisus haniltoaskin was possessed full of type I collagen resources,and acid-soluble method was more effective than pepsin-soluble method on extraction rate.Chemical crosslinking method could rise the denaturation temperature,thus the collagen from Pangasisus haniltoa skin could be the potential collagen resources,and it also could be used for the preparation of a variety of biomedical materials.It would own a wide application prospect.更多还原