【摘要】 目的对裸体方格星虫样品中脂肪酸类成分进行比较研究。方法采用气相色谱-质谱联用技术(GCMS)对12个裸体方格星虫样品中的26种脂肪酸进行了含量测定,并进一步对各样品中的各成分的含量进行了主成分分析。结果对裸体方格星虫样品的测定结果显示各样品26种脂肪酸类成分总含量范围为198.20~578.05μg/g,总含量最高的样品是海南三亚的裸体方格星虫体腔液样品(S12);各产地样品中脂肪酸类成分总含量均呈现体腔液部位高于外壁+内脏部位。测定样品的26种脂肪酸中,不饱和脂肪酸总含量高于饱和脂肪酸总含量;多不饱和脂肪酸中,亚油酸和α-亚麻酸含量最高,分别占总含量的1.54%~5.65%和1.61%~4.94%;单不饱和脂肪酸中顺芥子酸含量最高,分别占各样品总含量的14.66%~46.41%;基于26种脂肪酸的含量测定结果对12个裸体方格星虫样品的PCA分析结果显示不同产地裸体方格星虫的外壁+内脏样品相互接近,体腔液样品差别较大。结论裸体方格星虫中脂肪酸不仅种类多样、含量丰富,而且具有多种重要活性作用的不饱和脂肪酸比例合理。本研究中对脂肪酸类成分的研究结果将对进一步开发利用裸体方格星虫提供一定的理论基础。更多还原

【Abstract】 Objective To compare and evaluate fatty acids in Sipunculus nudus.Methods 26 fatty acids in12 batches of S.nudus samples were determined by GC-MS,and the data were further handled by a PCA scatter plot to compare the content variation of the samples.Results The total contents of 26 fatty acids in the samples ranged from 198.20μg/g(S9)to 578.05μg/g(S12),and the sample from Hainan had the highest total contents of 26 fatty acids.The total contents of 26 fatty acids in coelomic fluid were higher than those in body wall and intestine in S.nudus collected from all the regions.The total contents of unsaturated fatty acids were higher than saturated fatty acids.Among the polyunsaturated fatty acids,the contents of linoleic acid were between 1.54%and 5.65%and the contents ofα-linolenic acid were between 1.61% and 4.94%.The contents of cis-sinapic acid were the highest in monounsaturated fatty acids,which ranged from 14.66%to 46.41%.Moreover,the results of PCA performed on the basis of the contents of 26 tested compounds showed the difference of the parts in S.nudus samples.Conclusion S.nudus had an excellent source of fatty acids with reasonable proportion of unsaturated fatty acids.The research results provided a firm basis for further exploitation and development of S.nudus.更多还原